diff --git a/Marlin/src/module/thermistor/thermistor_21.h b/Marlin/src/module/thermistor/thermistor_21.h
index cd867df291..65bc17b3a5 100644
--- a/Marlin/src/module/thermistor/thermistor_21.h
+++ b/Marlin/src/module/thermistor/thermistor_21.h
@@ -23,10 +23,10 @@
 
 #define REVERSE_TEMP_SENSOR_RANGE_21 1
 
-#undef OV_SCALE
-#define OV_SCALE(N) (float((N) * 5) / 3.3f)
-
-// Pt100 with INA826 amp with 3.3v excitation based on "Pt100 with INA826 amp on Ultimaker v2.0 electronics"
+// Pt100 with INA826 amp with 3.3V excitation based on "Pt100 with INA826 amp on Ultimaker v2.0 electronics"
+// As the uC ADC reference voltage is 3.3V as well as the INA826 supply/reference, the same table as for 5V excitation is valid.
+// The ADC is referring his counts to the reference voltage as the IN826 opamp does scale the output voltage to the supply voltage in the analog domain.
+// Note, that the quantization error of this table in conjunction with rounding of the temperature reading can result in a few degrees error most noticeable between datapoints, e.g. 25°C, 35°C, etc.
 const temp_entry_t temptable_21[] PROGMEM = {
   { OV(  0),    0 },
   { OV(227),    1 },
@@ -73,5 +73,3 @@ const temp_entry_t temptable_21[] PROGMEM = {
   { OV(614),  500 }
 };
 
-#undef OV_SCALE
-#define OV_SCALE(N) (N)